Introduction
This blog to consider some important information when upgrading to BizTalk 2010
Upgrade roadmap
For BizTalk 2006 R2 and 2009 can be upgraded directly to BizTalk 2010:
But unfortunately upgrading directly from BizTalk 2006 to 2010 is not supported, so what you need to do in this case is to perform two stages upgrade:
Infrastructure Consideration
When upgrading to BizTalk 2010
BizTalk 2010 upgrade considerations:
References
i recommend to refer to this valuable documentation http://www.microsoft.com/en-us/download/details.aspx?id=11503
thank you